Hemagglutination-Inhibition Method and Immunofluorescence Staining with Venezuelan Equine Encephalomyelitis Virus

Hemagglutination-inhibition method and immunofluorescence staining with Venezuelan equine encephalomyelitis virus.

Hemagglutination and fluorescent antibody (FA) are compared for the direct detection of virus devoid of host cells. A determination was made of the minimal number of tissue plaque-forming units of Venezuelan equine encephalomyelitis virus that could be detected by the hemagglutination technique. Similar concentrations of the virus in bovine albumin borate saline, Brain Heart Infusion broth (Dif...

A slit sampler for collecting T-3 bacteriophage and Venezuelan equine encephalomyelitis virus. II. Studies with Venezuelan equine encephalomyelitis virus.

The virus employed was the fourth mouse brain passage of a strain of VEE virus recovered from a human infection in Colombia. The virus was suspended in phosphate buffer soluton (PBS),' pH 7.5, containing 2 per cent normal rabbit serum (NRS) to which antifoam A2 had been added to facilitate aerosolization. The virus suspension was aerosolized with a Collison3 generator in a modified Henderson4 a...

[Sedimentation characteristics of Venezuelan equine encephalomyelitis virus].

Morphogenesis of Venezuelan equine encephalomyelitis virus.

Morphogenesis of Venezuelan equine encephalomyelitis virus was studied by means of electron microscopy. Virus-specific structures (factories, viroplasts) were found at early stages of infection; these structures were composed of fibrillar and cylindrical formations, aggregates of ribosomes, and viral nucleoids. The latter emerged from fibrillar and cylindrical structures. Aggregates of viral nu...

Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus.

The conditions under which Venezuelan equine encephalomyelitis (VEE) virus attached to host cells markedly influenced the assay of virus by the fluorescent cell-counting technique. When virus inoculum was centrifuged onto McCoy cell monolayers, approximately 97% of virus was attached to cells within 10 min, in contrast to 34% after stationary incubation at 35 C for 2 hr. Maximal binding of viru...